Isolation, separation and purification of biomolecules

After biomass has been obtained, it is often necessary to disrupt the cells and extract compounds such as proteins or other biopolymers. Various homogenisation techniques are used that require different equipment which is available in the Industrial Microbiology Laboratories (Sonopuls mini 20, Bandelin). Furthermore, many techniques of characterisation only allow analysis of biomolecules purified to a certain degree. Thus, there are two preparative chromatography systems coupled with automated fraction collectors (Akta Pure 25, GE Healthcare) that allow molecules of interest to be separated from the bulk of cellular constituents.